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Addgene

EC.SurA.pTYB1.a21-428.M231R
(Plasmid #15956)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15956 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTYB1
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 7280
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mature SurA protein M231R mutant
  • Alt name
    SurA(M231R)
  • Species
    E. coli
  • Insert Size (bp)
    1224
  • Mutation
    M231R
  • Tag / Fusion Protein
    • intein (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is an additional mutation in this plasmid - M136I. Depositing scientist does not think it has any effect on protein function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EC.SurA.pTYB1.a21-428.M231R was a gift from David McKay (Addgene plasmid # 15956 ; http://n2t.net/addgene:15956 ; RRID:Addgene_15956)

  • For your References section:

    The Periplasmic Bacterial Molecular Chaperone SurA Adapts its Structure to Bind Peptides in Different Conformations to Assert a Sequence Preference for Aromatic Residues. Xu X, Wang S, Hu YX, McKay DB. J Mol Biol. 2007 Oct 19. 373(2):367-81. 10.1016/j.jmb.2007.07.069 PubMed 17825319
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