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R989-X28-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D
(Plasmid #159555)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159555 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDest-636
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D
  • Species
    H. sapiens (human)
  • Mutation
    G12D
  • Entrez Gene
    KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
  • Tag / Fusion Protein
    • His6-MBP-tev (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The SnapGene automatic annotation of K-Ras mistakenly suggests there is a G12V mutation. The true mutation in the K-Ras sequence is G12D.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R989-X28-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D was a gift from Dominic Esposito (Addgene plasmid # 159555 ; http://n2t.net/addgene:159555 ; RRID:Addgene_159555)

  • For your References section:

    Farnesylated and methylated KRAS4b: high yield production of protein suitable for biophysical studies of prenylated protein-lipid interactions. Gillette WK, Esposito D, Abreu Blanco M, Alexander P, Bindu L, Bittner C, Chertov O, Frank PH, Grose C, Jones JE, Meng Z, Perkins S, Van Q, Ghirlando R, Fivash M, Nissley DV, McCormick F, Holderfield M, Stephen AG. Sci Rep. 2015 Nov 2;5:15916. doi: 10.1038/srep15916. 10.1038/srep15916 PubMed 26522388
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