pM12-ISH
(Plasmid
#15954)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15954 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBSIIKS-
- Backbone size w/o insert (bp) 3000
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameM12
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Alt nameMOR140-1
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Alt nameolfr19
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SpeciesM. musculus (mouse)
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Insert Size (bp)1441
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GenBank IDAY073758
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Entrez GeneOlfr19 (a.k.a. M12, MOR140-1, MTPCR15)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer M13 Forward
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
to make RNA probe, cut with SpeI. Then do in vitro transcription with T3RNA polymerase.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pM12-ISH was a gift from Peter Mombaerts (Addgene plasmid # 15954 ; http://n2t.net/addgene:15954 ; RRID:Addgene_15954) -
For your References section:
Local and cis Effects of the H Element on Expression of Odorant Receptor Genes in Mouse. Fuss SH, Omura M, Mombaerts P. Cell. 2007 Jul 27. 130(2):373-84. 10.1016/j.cell.2007.06.023 PubMed 17662950