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pQE30-VRN1
(Plasmid #159531)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159531 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE30
  • Backbone manufacturer
    Qiagen
  • Backbone size w/o insert (bp) 3433
  • Total vector size (bp) 3845
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    VERNALIZATION1
  • Alt name
    VRN1
  • Alt name
    At3g18990
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    411
  • Entrez Gene
    VRN1 (a.k.a. AT3G18990, REDUCED VERNALIZATION RESPONSE 1, REM39, REPRODUCTIVE MERISTEM 39)
  • Promoter T5
  • Tag / Fusion Protein
    • Six-Histidine tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CCCGAAAAGTGCCACCTG
  • 3′ sequencing primer GTTCTGAGGTCATTACTGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE30-VRN1 was a gift from Joshua Mylne (Addgene plasmid # 159531 ; http://n2t.net/addgene:159531 ; RRID:Addgene_159531)

  • For your References section:

    The Arabidopsis B3 domain protein VERNALIZATION1 (VRN1) is involved in processes essential for development, with structural and mutational studies revealing its DNA-binding surface. King GJ, Chanson AH, McCallum EJ, Ohme-Takagi M, Byriel K, Hill JM, Martin JL, Mylne JS. J Biol Chem. 2013 Feb 1;288(5):3198-207. doi: 10.1074/jbc.M112.438572. Epub 2012 Dec 19. 10.1074/jbc.M112.438572 PubMed 23255593
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