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pQE30-HaAEP1
(Plasmid #159530)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159530 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE30
  • Backbone manufacturer
    Qiagen
  • Backbone size w/o insert (bp) 3443
  • Total vector size (bp) 4837
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Helianthus annuus asparaginyl endopeptidase 1
  • Alt name
    HaAEP1
  • Species
    Synthetic
  • Insert Size (bp)
    1404
  • GenBank ID
    KJ147147
  • Entrez Gene
    LOC110922574 (a.k.a. HannXRQ_Chr17g0548711, AEP1, VPE)
  • Promoter T5
  • Tag / Fusion Protein
    • Six-Histidine tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CCCGAAAAGTGCCACCTG
  • 3′ sequencing primer GTTCTGAGGTCATTACTGG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Geneart

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Recommend propagation either in combination with QIAGEN plasmid pREP4 or in a E. coli strain with high lacI expression (e.g Novablue)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE30-HaAEP1 was a gift from Joshua Mylne (Addgene plasmid # 159530 ; http://n2t.net/addgene:159530 ; RRID:Addgene_159530)

  • For your References section:

    Peptide macrocyclization by a bifunctional endoprotease. Bernath-Levin K, Nelson C, Elliott AG, Jayasena AS, Millar AH, Craik DJ, Mylne JS. Chem Biol. 2015 May 21;22(5):571-82. doi: 10.1016/j.chembiol.2015.04.010. Epub 2015 May 7. 10.1016/j.chembiol.2015.04.010 PubMed 25960260
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