pQE30-TEV-pro-conA
(Plasmid #159527)

• Purpose: Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.
• Depositing Lab: Joshua Mylne
• Publication: Nonis et al bioRxiv. 2020.

Backbone

• Vector backbone: pQE30
• Backbone manufacturer: QIAGEN
• Backbone size w/o insert (bp): 3434
• Total vector size (bp): 4247
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Canavalia ensiformis pro-concanavalin A
• Alt name: pro-conA
• Species: Synthetic
• Insert Size (bp): 813
• GenBank ID: AF308777
• Promoter: T5
• Tags / Fusion Proteins:
  • Six-Histidine tag (N terminal on backbone)
  • tobacco etch virus (TEV) protease recognition site (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CCCGAAAAGTGCCACCTG
• 3′ sequencing primer: GTTCTGAGGTCATTACTGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.10.28.360099v1 for bioRxiv preprint.