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pAAV-LAP1-mCherry
(Plasmid #159525)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159525 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-Ef1a-mCherry (Addgene 114470)
  • Backbone manufacturer
    Karl Deisseroth and Stratagene
  • Backbone size w/o insert (bp) 4566
  • Total vector size (bp) 5277
  • Modifications to backbone
    Replaced EF1a promoter with LAP1
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    711
  • Promoter LAP1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GCA CGC GTA TCT CCG GAA AGA GGA AAT TGA
  • 3′ sequencing primer GCG GAT CCT ATA TAC ACG ATG TGC ATC CAT AAT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-LAP1-mCherry was a gift from Esteban Engel (Addgene plasmid # 159525 ; http://n2t.net/addgene:159525 ; RRID:Addgene_159525)

  • For your References section:

    Small Alphaherpesvirus Latency-Associated Promoters Drive Efficient and Long-Term Transgene Expression in the CNS. Maturana CJ, Verpeut JL, Pisano TJ, Dhanerawala ZM, Esteves A, Enquist LW, Engel EA. Mol Ther Methods Clin Dev. 2020 Apr 14;17:843-857. doi: 10.1016/j.omtm.2020.04.004. eCollection 2020 Jun 12. 10.1016/j.omtm.2020.04.004 PubMed 32368565
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