pAAV-LAP2-mCherry
(Plasmid
#159524)
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PurposeExpresses mCherry from LAP2 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159524 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-Ef1a-mCherry (Addgene 114470)
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Backbone manufacturerKarl Deisseroth and Stratagene
- Backbone size w/o insert (bp) 4469
- Total vector size (bp) 5180
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Modifications to backboneReplaced EF1a promoter with LAP2
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry
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Insert Size (bp)711
- Promoter LAP2
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer GCA CGC GTA TCC CCG GTC CGC GCT CCG CCC ACC CA
- 3′ sequencing primer GCG GAT CCG AGC TCC CTC TTC CTC GCC GCG GAC TGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-LAP2-mCherry was a gift from Esteban Engel (Addgene plasmid # 159524 ; http://n2t.net/addgene:159524 ; RRID:Addgene_159524) -
For your References section:
Small Alphaherpesvirus Latency-Associated Promoters Drive Efficient and Long-Term Transgene Expression in the CNS. Maturana CJ, Verpeut JL, Pisano TJ, Dhanerawala ZM, Esteves A, Enquist LW, Engel EA. Mol Ther Methods Clin Dev. 2020 Apr 14;17:843-857. doi: 10.1016/j.omtm.2020.04.004. eCollection 2020 Jun 12. 10.1016/j.omtm.2020.04.004 PubMed 32368565