pAAV-LAP2-mCherry
(Plasmid #159524)

• Purpose: Expresses mCherry from LAP2 promoter
• Depositing Lab: Esteban Engel
• Publication: Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;17:843-857. doi: 10.1016/j.omtm.2020.04.004. eCollection 2020 Jun 12.

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Backbone

• Vector backbone: pAAV-Ef1a-mCherry (Addgene 114470)
• Backbone manufacturer: Karl Deisseroth and Stratagene
• Backbone size w/o insert (bp): 4469
• Total vector size (bp): 5180
• Modifications to backbone: Replaced EF1a promoter with LAP2
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry
• Insert Size (bp): 711
• Promoter: LAP2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: GCA CGC GTA TCC CCG GTC CGC GCT CCG CCC ACC CA
• 3′ sequencing primer: GCG GAT CCG AGC TCC CTC TTC CTC GCC GCG GAC TGG

Resource Information

• Supplemental Documents:
  • pVC 144.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry