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Addgene

pJO818 (pRS425 Gal1-Ufd2-flag)
(Plasmid #159522)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159522 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS425-Gal1
  • Backbone size w/o insert (bp) 7540
  • Total vector size (bp) 10453
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ufd2
  • Alt name
    YDL190C
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2886
  • Promoter Gal1
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13-F
  • 3′ sequencing primer M13-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJO818 (pRS425 Gal1-Ufd2-flag) was a gift from Alexander Varshavsky (Addgene plasmid # 159522 ; http://n2t.net/addgene:159522 ; RRID:Addgene_159522)
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