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Addgene

pM71-ISH
(Plasmid #15947)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15947 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBSIISK-
  • Backbone size w/o insert (bp) 3000

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    M71
  • Alt name
    MOR171-2
  • Alt name
    Olfr1511
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    905
  • GenBank ID
    AF281061
  • Entrez Gene
    Olfr151 (a.k.a. M71, MOR171-2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site KpnI (destroyed during cloning)
  • 5′ sequencing primer M13 reverse
  • 3′ sequencing primer M13 forward
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

to make RNA probe, cut with SacI. Then do in vitro transcription with T7RNA polymerase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pM71-ISH was a gift from Peter Mombaerts (Addgene plasmid # 15947 ; http://n2t.net/addgene:15947 ; RRID:Addgene_15947)
  • For your References section:

    Local and cis Effects of the H Element on Expression of Odorant Receptor Genes in Mouse. Fuss SH, Omura M, Mombaerts P. Cell. 2007 Jul 27. 130(2):373-84. 10.1016/j.cell.2007.06.023 PubMed 17662950