pET15-mFAP10
(Plasmid #159463)

• Purpose: Encodes for mFAP10 with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
• Depositing Lab: David Baker
• Publication: Baker Lab mFAP plasmids (unpublished)

Backbone

• Vector backbone: pET15
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mFAP10
• Species: E. coli
• Tag / Fusion Protein:
  • 6xHis-thrombin-TEV (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pET15-mFAP10.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry