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Addgene

mCRISPRed pRSETB
(Plasmid #159454)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159454 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSETB
  • Backbone manufacturer
    Invitrogen/Thermofisher Scientific
  • Backbone size w/o insert (bp) 2901
  • Total vector size (bp) 3609
  • Modifications to backbone
    Increased His-Tag length to 10 x from the original 6 x
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCRISPRed
  • Species
    Entacmaea quadricolor
  • Insert Size (bp)
    708
  • GenBank ID
    MN844880.1 MN844880.1
  • Promoter T7
  • Tag / Fusion Protein
    • 10 x His-Tag (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGG
  • 3′ sequencing primer GCTTCCTTTCGGGCTTTGTTAGCAGCCGGATC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCRISPRed pRSETB was a gift from Oliver Griesbeck (Addgene plasmid # 159454 ; http://n2t.net/addgene:159454 ; RRID:Addgene_159454)

  • For your References section:

    Targeted In Situ Protein Diversification and Intra-organelle Validation in Mammalian Cells. Erdogan M, Fabritius A, Basquin J, Griesbeck O. Cell Chem Biol. 2020 May 21;27(5):610-621.e5. doi: 10.1016/j.chembiol.2020.02.004. Epub 2020 Mar 5. 10.1016/j.chembiol.2020.02.004 PubMed 32142629
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