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Addgene

pCL66-decoder
(Plasmid #159453)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159453 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRG235
  • Backbone manufacturer
    addgene
  • Backbone size w/o insert (bp) 3939
  • Total vector size (bp) 6874
  • Vector type
    Yeast Expression, Synthetic Biology
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pfus1mut-tetR-nls-malE-tCyc1
  • Species
    Synthetic
  • Insert Size (bp)
    2935
  • Promoter Pfus1mut

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer AATTAACCCTCACTAAAGGG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Part of the insert (tetr-nls-malE) from Azizoğlu, A., Brent, R. & Rudolf, F. A precisely-titratable, variation-suppressed transcriptional controller to enable genetic discovery. bioRxiv, doi:10.1101/2019.12.12.874461 (2019).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCL66-decoder was a gift from Joerg Stelling (Addgene plasmid # 159453 ; http://n2t.net/addgene:159453 ; RRID:Addgene_159453)

  • For your References section:

    A rationally engineered decoder of transient intracellular signals. Lormeau C, Rudolf F, Stelling J. Nat Commun. 2021 Mar 25;12(1):1886. doi: 10.1038/s41467-021-22190-4. 10.1038/s41467-021-22190-4 PubMed 33767179
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