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pSB_init_Sb68
(Plasmid #159423)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159423 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSB_init
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Sb68
  • Species
    Synthetic
  • Tag / Fusion Protein
    • c-Myc and poly-His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer pBAD Forward
  • 3′ sequencing primer pBAD Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full sequence is provided but the depositors have only verified the sequence of the multi-cloning site and the TGP-encoding region by DNA sequencing.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSB_init_Sb68 was a gift from Dianfan Li (Addgene plasmid # 159423 ; http://n2t.net/addgene:159423 ; RRID:Addgene_159423)

  • For your References section:

    An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification. Cai H, Yao H, Li T, Hutter CAJ, Li Y, Tang Y, Seeger MA, Li D. Commun Biol. 2020 Dec 10;3(1):753. doi: 10.1038/s42003-020-01478-z. 10.1038/s42003-020-01478-z PubMed 33303987
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