pSB_init-Sb44
(Plasmid
#159421)
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PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb44
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Depositing Lab
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Sequence Information
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Sequences (3) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159421 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSB_init
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSb44
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SpeciesSynthetic
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Tag
/ Fusion Protein
- c-Myc and poly-His (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer pBAD Forward
- 3′ sequencing primer pBAD Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Full sequence is provided but the depositors have only verified the sequence of the sybody-encoding region by DNA sequencing.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSB_init-Sb44 was a gift from Dianfan Li (Addgene plasmid # 159421 ; http://n2t.net/addgene:159421 ; RRID:Addgene_159421) -
For your References section:
An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification. Cai H, Yao H, Li T, Hutter CAJ, Li Y, Tang Y, Seeger MA, Li D. Commun Biol. 2020 Dec 10;3(1):753. doi: 10.1038/s42003-020-01478-z. 10.1038/s42003-020-01478-z PubMed 33303987
