DT042A
(Plasmid #159403)

• Purpose: Inducible superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter
• Depositing Lab: Sheref Mansy
• Publication: Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/eabb4920. doi: 10.1126/sciadv.abb4920. Print 2020 Sep.

Backbone

• Vector backbone: pET21b(+)
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: pr.T5-LacO-6xHis-sfGFP
• Species: Synthetic
• Promoter: T5 promoter
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • DT042A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: This plasmid exists as a multimer. This is not expected to impact the end function of the plasmid. Please refer to the Addgene Blog post on multimers for more information: https://blog.addgene.org/plasmids-101-dimers-and-multimers