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Addgene

DT033A
(Plasmid #159394)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159394 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSB1A3
  • Backbone manufacturer
    iGEM parts registry
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pr.tac-BDNF (Bdnf, Mouse)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    624
  • Entrez Gene
    Bdnf
  • Promoter tac promoter
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer VF2 primer (iGEM parts registry)
  • 3′ sequencing primer VR primer (iGEM parts registry)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DT033A was a gift from Sheref Mansy (Addgene plasmid # 159394 ; http://n2t.net/addgene:159394 ; RRID:Addgene_159394)

  • For your References section:

    Artificial cells drive neural differentiation. Toparlak OD, Zasso J, Bridi S, Serra MD, Macchi P, Conti L, Baudet ML, Mansy SS. Sci Adv. 2020 Sep 18;6(38). pii: 6/38/eabb4920. doi: 10.1126/sciadv.abb4920. Print 2020 Sep. 10.1126/sciadv.abb4920 PubMed 32948587
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