Mouse Toronto KnockOut (mTKO) CRISPR Library
(Pooled Libraries #159392, #159393)
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Purpose
Mouse lentiviral genome-wide CRISPR library targets protein-coding genes with ~5 gRNAs/gene.
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Vector Backbone
- lentiCRISPR v2 (Plasmid #52961) backbone (one-vector system) - expresses Cas9
- pLCKO2 (Plasmid #125518) backbone (two-vector system) - does not express Cas9
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Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Pooled Library | 159392 | gRNA pooled library in lentiCRISPR v2 | 1 | $540 | Add to Cart | |
| Pooled Library | 159393 | gRNA pooled library in pLCKO2 | 1 | $540 | Add to Cart | |
Library Details
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SpeciesMouse
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Genes targeted19,463
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gRNAs94,528
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Controls418 control gRNAs targeting EGFP, LacZ, and Luciferase
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Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
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Volume∼20 µL
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Concentration50 ng/µL
Resource Information
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Protocols
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Depositor Data
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Articles Citing this Pooled Library
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Terms and Licenses
Academic/Nonprofit TermsIndustry Terms- Not Available to Industry
Trademarks- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositing lab recommends using Bayesian Analysis of Gene Essentiality (BAGEL) (Link opens in a new window) to analyze fold changes. The MAGeCK/MAGeCK-VISPR algorithms (Link opens in a new window) can also be used for analysis.
The DrugZ algorithm (Link opens in a new window) is recommended for drug screens.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Mouse Toronto KnockOut (mTKO) CRISPR library in lentiCRISPR v2 was a gift from Jason Moffat (Addgene #159392)
Mouse Toronto KnockOut (mTKO) CRISPR library in pLCKO2 was a gift from Jason Moffat (Addgene #159393) -
For your References section:
Functional genomic landscape of cancer-intrinsic evasion of killing by T cells. Lawson KA, Sousa CM, Zhang X, Kim E, Akthar R, Caumanns JJ, Yao Y, Mikolajewicz N, Ross C, Brown KR, Zid AA, Fan ZP, Hui S, Krall JA, Simons DM, Slater CJ, De Jesus V, Tang L, Singh R, Goldford JE, Martin S, Huang Q, Francis EA, Habsid A, Climie R, Tieu D, Wei J, Li R, Tong AHY, Aregger M, Chan KS, Han H, Wang X, Mero P, Brumell JH, Finelli A, Ailles L, Bader G, Smolen GA, Kingsbury GA, Hart T, Kung C, Moffat J. Nature. 2020 Oct;586(7827):120-12. doi: 10.1038/s41586-020-2746-2. PubMed 32968282 (Link opens in a new window)