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Addgene

pET28a 6xHis PreScission SARS-CoV-2 nsp13 Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pET28a 6xHis PreScission SARS-CoV-2 nsp13 with the following enzyme(s): Nde I, PvuI-HF, and SalI-HF.

Expected bands:
Nde I/SalI-HF 5503 + 1601 bp
PvuI-HF 7104 bp

Results:
We observed the expected bands for both digests.