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Addgene

pET-gSTEP1
(Plasmid #159376)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159376 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-Duet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5301
  • Total vector size (bp) 6141
  • Modifications to backbone
    Deletion of most of MCS1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gSTEP1
  • Species
    Synthetic
  • Insert Size (bp)
    840
  • Promoter T7
  • Tag / Fusion Protein
    • 6x His tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer DuetUP2 TTGTACACGGCCGCATAATC
  • 3′ sequencing primer T7 Terminal GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-gSTEP1 was a gift from Roberto Chica (Addgene plasmid # 159376 ; http://n2t.net/addgene:159376 ; RRID:Addgene_159376)

  • For your References section:

    Genetically Encoded Fluorescent Biosensor for Rapid Detection of Protein Expression. Eason MG, Pandelieva AT, Mayer MM, Khan ST, Garcia HG, Chica RA. ACS Synth Biol. 2020 Nov 20;9(11):2955-2963. doi: 10.1021/acssynbio.0c00407. Epub 2020 Oct 12. 10.1021/acssynbio.0c00407 PubMed 33044070
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