pCAGGS-SARS2-S-FKO (NC-flag)
(Plasmid #159363)

• Purpose: Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.
• Depositing Labs: Hyeryun Choe, Michael Farzan
• Publication: Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi: 10.1038/s41467-020-19808-4.

Backbone

• Vector backbone: pCAGGS
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SARS-CoV-2 S protein
• Species: SARS-CoV-2
• Insert Size (bp): 3900
• Mutation: Furin-cleavage site mutation
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SbfI (not destroyed)
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pCAGGS-SARS2-S-FKO (NC-flag).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Pseudotypes to the MLV vector but not efficiently to VSV or lentiviral vectors.

Please visit https://www.biorxiv.org/content/10.1101/2020.06.12.148726v1 for bioRxiv preprint.