pCRII-BbsI-sgRNAscaffold
(Plasmid
#159352)
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Purpose(Empty Backbone) Expression vector for a customizable guide RNA
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159352 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCRII
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Backbone manufacturerThermo Fisher Scientific
- Backbone size (bp) 4426
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Vector typeMammalian Expression, CRISPR
- Promoter U6
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 rev
- 3′ sequencing primer M13 fw (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCRII-BbsI-sgRNAscaffold was a gift from Pim Pijnappel (Addgene plasmid # 159352 ; http://n2t.net/addgene:159352 ; RRID:Addgene_159352)
