pISH-cre
(Plasmid
#15933)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15933 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM-T Easy
- Backbone size w/o insert (bp) 3015
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecre recombinase
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Insert Size (bp)1031
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Mutation642C to T, 966A to G
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Entrez Genecre (a.k.a. P1_gp003)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
NcoI digestion and SP6 RNA polymerase for antisense probe
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pISH-cre was a gift from Peter Mombaerts (Addgene plasmid # 15933 ; http://n2t.net/addgene:15933 ; RRID:Addgene_15933) -
For your References section:
Odorant receptor gene choice is reset by nuclear transfer from mouse olfactory sensory neurons. Li J, Ishii T, Feinstein P, Mombaerts P. Nature. 2004 Mar 25. 428(6981):393-9. 10.1038/nature02433 PubMed 15042081