pcDNA3-3N-Flag-mLIG3-178
(Plasmid
#159315)
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PurposeMammalian cell expression of mouse LIG3-178
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159315 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemLIG3-178
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SpeciesM. musculus (mouse)
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Entrez GeneLig3 (a.k.a. D11Wsu78, D11Wsu78e)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains a S36T mutation which falls in the linker upstream of mLIG3. It is not known how this mutation affects plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-3N-Flag-mLIG3-178 was a gift from Jianjun Zhao (Addgene plasmid # 159315 ; http://n2t.net/addgene:159315 ; RRID:Addgene_159315)