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Addgene

pJH23
(Plasmid #159314)

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Item Catalog # Description Quantity Price (USD)
Plasmid 159314 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pSL1180
  • Total vector size (bp) 11874
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    syd-2
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    5307
  • Entrez Gene
    syd-2 (a.k.a. CELE_F59F5.6)
  • Promoter Punc-25
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer gcggccgcgaaatatcgatt
  • 3′ sequencing primer ggatccgCACAGCATCACTTTCGTCA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJH23 was a gift from Mei Zhen (Addgene plasmid # 159314 ; http://n2t.net/addgene:159314 ; RRID:Addgene_159314)

  • For your References section:

    Identification of genes involved in synaptogenesis using a fluorescent active zone marker in Caenorhabditis elegans. Yeh E, Kawano T, Weimer RM, Bessereau JL, Zhen M. J Neurosci. 2005 Apr 13;25(15):3833-41. doi: 10.1523/JNEUROSCI.4978-04.2005. 10.1523/JNEUROSCI.4978-04.2005 PubMed 15829635
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