pKLV2-U6gRNA5(gKAT7-A10)-PGKpuro2ABFP-W
(Plasmid #159288)

• Purpose: Lentiviral vector expressing gRNA targeting KAT7 (gRNA ID. A10)
• Depositing Lab: Kosuke Yusa
• Publication: Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi: 10.1038/s41375-020-1001-z. Epub 2020 Aug 6.

Backbone

• Vector backbone: pKLV2-U6gRNA5(BbsI)-PGKpuroBFP-W
• Backbone size w/o insert (bp): 8630
• Total vector size (bp): 8650
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: guide RNA targeting KAT7 (ID. A10)
• gRNA/shRNA sequence: GCCGCTATGAGCTTGATACC
• Species: H. sapiens (human)
• GenBank ID: NM_007067
• Entrez Gene: KAT7 (a.k.a. HBO1, HBOA, MYST2, ZC2HC7)
• Promoter: human U6 promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (destroyed during cloning)
• 3′ cloning site: BbsI (destroyed during cloning)
• 5′ sequencing primer: AGATAATTAGAATTAATTTGACTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.04.25.054049v1 for bioRxiv preprint.