HA-EcPPK1
(Plasmid
#159279)
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PurposeExpresses a HA-tagged E. coli PPK1 in mammalian cell lines
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159279 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1(-)
- Backbone size w/o insert (bp) 5427
- Total vector size (bp) 7508
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHA-tagged Polyphosphate Kinase 1
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Alt namePPK1
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SpeciesEscherichia coli
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Insert Size (bp)2103
- Promoter CMV
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer tccaccacactggactagtgATGTACCCATACGATGTTC
- 3′ sequencing primer tgatcagcggtttaaacttaTTATTCAGGTTGTTCGAG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA-EcPPK1 was a gift from Michael Downey (Addgene plasmid # 159279 ; http://n2t.net/addgene:159279 ; RRID:Addgene_159279) -
For your References section:
A Broad Response to Intracellular Long-Chain Polyphosphate in Human Cells. Bondy-Chorney E, Abramchuk I, Nasser R, Holinier C, Denoncourt A, Baijal K, McCarthy L, Khacho M, Lavallee-Adam M, Downey M. Cell Rep. 2020 Oct 27;33(4):108318. doi: 10.1016/j.celrep.2020.108318. 10.1016/j.celrep.2020.108318 PubMed 33113373
