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Addgene

HA-EcPPK1
(Plasmid #159279)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159279 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone size w/o insert (bp) 5427
  • Total vector size (bp) 7508
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HA-tagged Polyphosphate Kinase 1
  • Alt name
    PPK1
  • Species
    Escherichia coli
  • Insert Size (bp)
    2103
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tccaccacactggactagtgATGTACCCATACGATGTTC
  • 3′ sequencing primer tgatcagcggtttaaacttaTTATTCAGGTTGTTCGAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-EcPPK1 was a gift from Michael Downey (Addgene plasmid # 159279 ; http://n2t.net/addgene:159279 ; RRID:Addgene_159279)

  • For your References section:

    A Broad Response to Intracellular Long-Chain Polyphosphate in Human Cells. Bondy-Chorney E, Abramchuk I, Nasser R, Holinier C, Denoncourt A, Baijal K, McCarthy L, Khacho M, Lavallee-Adam M, Downey M. Cell Rep. 2020 Oct 27;33(4):108318. doi: 10.1016/j.celrep.2020.108318. 10.1016/j.celrep.2020.108318 PubMed 33113373
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