Skip to main content
Addgene

pET11a-DAAT-V33G/T242G
(Plasmid #159278)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159278 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET-11a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5677
  • Total vector size (bp) 6517
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Engineered d-alanine aminotransferase
  • Alt name
    DAAT-V33G/T242G
  • Species
    Bacillus sp.
  • Insert Size (bp)
    876
  • Mutation
    Valine 33 and threonine 242 have been mutated to glycine
  • GenBank ID
    J04460.1
  • Promoter T7
  • Tag / Fusion Protein
    • 6x His tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 TAATACGACTCACTATAGGG
  • 3′ sequencing primer T7 Terminal GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET11a-DAAT-V33G/T242G was a gift from Roberto Chica (Addgene plasmid # 159278 ; http://n2t.net/addgene:159278 ; RRID:Addgene_159278)

  • For your References section:

    One-Pot Biocatalytic Synthesis of Substituted D-Tryptophans from Indoles Enabled by an Engineered Aminotransferase. Parmeggiani F, Rué Casamajo A, Walton CJW, Galman JL, Turner NJ, Chica RA. ACS Catalysis 9, 3482–3486. 10.1021/acscatal.9b00739
Related items: