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Addgene

AAVS1-tdTomato
(Plasmid #159275)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159275 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Vector type
    Mammalian Expression, AAV ; S1 knockin donor vector
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tdTomato
  • Insert Size (bp)
    1431
  • Promoter CAGGS

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer ggcggctctagagcctctgctaacc
  • 3′ sequencing primer ttggcagagggaaaaagatctcagtgg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1-tdTomato was a gift from Rudolf Jaenisch (Addgene plasmid # 159275 ; http://n2t.net/addgene:159275 ; RRID:Addgene_159275)

  • For your References section:

    Establishment of human pluripotent stem cell-derived pancreatic beta-like cells in the mouse pancreas. Ma H, Wert KJ, Shvartsman D, Melton DA, Jaenisch R. Proc Natl Acad Sci U S A. 2018 Apr 10;115(15):3924-3929. doi: 10.1073/pnas.1702059115. Epub 2018 Mar 29. 10.1073/pnas.1702059115 PubMed 29599125
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