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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15925 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMSCVgfp
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 6543
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAID
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Alt nameAICDA
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Alt nameactivation-induced cytidine deaminase
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SpeciesH. sapiens (human)
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Insert Size (bp)594
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GenBank IDNM_009645
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Entrez GeneAICDA (a.k.a. AID, ARP2, CDA2, HEL-S-284, HIGM2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer MSCV (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCVgfp::AID was a gift from Nina Papavasiliou (Addgene plasmid # 15925 ; http://n2t.net/addgene:15925 ; RRID:Addgene_15925) -
For your References section:
AID mediates hypermutation by deaminating single stranded DNA. Dickerson SK, Market E, Besmer E, Papavasiliou FN. J Exp Med. 2003 May 19. 197(10):1291-6. 10.1084/jem.20030481 PubMed 12756266