pET3d::strep-AID
(Plasmid #15924)

• Depositing Lab: Nina Papavasiliou
• Publication: Dickerson et al J Exp Med. 2003 May 19. 197(10):1291-6.

Backbone

• Vector backbone: pET3d
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 4657
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AID
• Alt name: AICDA
• Alt name: activation-induced cytidine deaminase
• Species: H. sapiens (human)
• Insert Size (bp): 594
• GenBank ID: NM_009645
• Entrez Gene: AICDA (a.k.a. AID, ARP2, CDA2, HEL-S-284, HIGM2)
• Tag / Fusion Protein:
  • strep

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Has a point mutation resulting in N103Y substitution in the amino acid sequence. According to depositing scientist, the mutation does not affect function.