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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15923 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5350
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAID
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Alt nameAICDA
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Alt nameactivation-induced cytidine deaminase
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SpeciesM. musculus (mouse)
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Insert Size (bp)594
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GenBank IDNM_009645
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Entrez GeneAicda (a.k.a. Aid, Arp2)
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Tag
/ Fusion Protein
- His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28b::mAID was a gift from Nina Papavasiliou (Addgene plasmid # 15923 ; http://n2t.net/addgene:15923 ; RRID:Addgene_15923) -
For your References section:
The transcription elongation complex directs activation-induced cytidine deaminase-mediated DNA deamination. Besmer E, Market E, Papavasiliou FN. Mol Cell Biol. 2006 Jun . 26(11):4378-85. 10.1128/MCB.02375-05 PubMed 16705187