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Addgene

pET28b::mAID
(Plasmid #15923)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15923 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5350
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AID
  • Alt name
    AICDA
  • Alt name
    activation-induced cytidine deaminase
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    594
  • GenBank ID
    NM_009645
  • Entrez Gene
    Aicda (a.k.a. Aid, Arp2)
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b::mAID was a gift from Nina Papavasiliou (Addgene plasmid # 15923 ; http://n2t.net/addgene:15923 ; RRID:Addgene_15923)
  • For your References section:

    The transcription elongation complex directs activation-induced cytidine deaminase-mediated DNA deamination. Besmer E, Market E, Papavasiliou FN. Mol Cell Biol. 2006 Jun . 26(11):4378-85. 10.1128/MCB.02375-05 PubMed 16705187