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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15922 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneptac
- Backbone size w/o insert (bp) 6064
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsGrows only in ccdB resistant strains
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameccdB
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SpeciesE. coli
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Insert Size (bp)303
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Entrez GeneccdB (a.k.a. pCoo051)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer tac promoter (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ptac::ccdB was a gift from Nina Papavasiliou (Addgene plasmid # 15922 ; http://n2t.net/addgene:15922 ; RRID:Addgene_15922) -
For your References section:
The transcription elongation complex directs activation-induced cytidine deaminase-mediated DNA deamination. Besmer E, Market E, Papavasiliou FN. Mol Cell Biol. 2006 Jun . 26(11):4378-85. 10.1128/MCB.02375-05 PubMed 16705187