pLV-mScarlet-I
(Plasmid #159172)

• Purpose: To express a bright monomeric red FP for labelling and detection of mammalian cells
• Depositing Lab: Pantelis Tsoulfas
• Publication: Pantelis Tsoulfas Lab plasmid (unpublished)

Backbone

• Vector backbone: pLenti-MP2
• Backbone manufacturer: Naldini Lab
• Backbone size w/o insert (bp): 6782
• Total vector size (bp): 7497
• Vector type: Mammalian Expression, Lentiviral, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: optimal growth in low salt LB (5gm NaCl)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mScarlet-I
• Insert Size (bp): 700
• Mutation: Codons were optimized to Human codon usage
• GenBank ID: KY021424.1
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV fwd
• 3′ sequencing primer: WPRE-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The codons were optimized to the Human codon usage