pCDFDuet-1::ADAT3-ADAT2
(Plasmid #15917)

• Depositing Lab: Nina Papavasiliou
• Publication: Rubio et al Proc Natl Acad Sci U S A. 2007 May 8. 104(19):7821-6.

Backbone

• Vector backbone: pCDFDuet-1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3781
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ADAT3 & ADAT2
• Species: T. brucei
• Insert Size (bp): 1097
• Tags / Fusion Proteins:
  • HexaHistidine (N terminal on backbone)
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI & NdeI (not destroyed)
• 3′ cloning site: NotI & XhoI (not destroyed)
• 5′ sequencing primer: sequence
• 3′ sequencing primer: sequence

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry