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pcDNA3-HA-DEPTOR S286/287/291A
(Plasmid #159160)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159160 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6630
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DEPTOR
  • Alt name
    DEPDC6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1230
  • Mutation
    changed serine 286,287 and 291 to alanine
  • GenBank ID
    NM_022783.4
  • Entrez Gene
    DEPTOR (a.k.a. DEP.6, DEPDC6, hDEPTOR)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA-DEPTOR S286/287/291A was a gift from Michele Pagano (Addgene plasmid # 159160 ; http://n2t.net/addgene:159160 ; RRID:Addgene_159160)

  • For your References section:

    mTOR generates an auto-amplification loop by triggering the betaTrCP- and CK1alpha-dependent degradation of DEPTOR. Duan S, Skaar JR, Kuchay S, Toschi A, Kanarek N, Ben-Neriah Y, Pagano M. Mol Cell. 2011 Oct 21;44(2):317-24. doi: 10.1016/j.molcel.2011.09.005. 10.1016/j.molcel.2011.09.005 PubMed 22017877
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