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Addgene

LTRi EGFP
(Plasmid #15914)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15914 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    LTRi (with neomycin R)
  • Backbone size w/o insert (bp) 11100
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Stratagene SURE Very low copy: TLD recommends Megapreps
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Insert Size (bp)
    800

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We engineered a tunable genetic switch called LTRi, which stands for Lac-Tet-RNAi. Gene expression is turned on by adding an inducer, isopropyl-β-thiogalactopyranoside (IPTG). LTRi-EGFP allows scientists to tune the level of EGFP. See article for details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LTRi EGFP was a gift from James Collins (Addgene plasmid # 15914)

  • For your References section:

    A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells. Deans TL, Cantor CR, Collins JJ. Cell. 2007 Jul 27. 130(2):363-72. 10.1016/j.cell.2007.05.045 PubMed 17662949
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