pCMV3TAG8li-hRNF160-3FLAG
(Plasmid #159138)

• Purpose: human RNF160 (LTN1, ZNF294) FLAG mammalian expression vector.
• Depositing Lab: Martin Dorf
• Publication: Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub 2011 Sep 8.

Backbone

• Vector backbone: pCMV-3Tag-8
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 5200
• Modifications to backbone: MCS replaced with new restriction sites: XhoI, HindIII, BamHI, NotI, ClaI
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: listerin E3 ubiquitin protein ligase 1
• Alt name: LTN1; RNF160; ZNF294; C21orf10; C21orf98
• Species: H. sapiens (human)
• Entrez Gene: LTN1 (a.k.a. C21orf10, C21orf98, RNF160, ZNF294)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xFLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: ClaI (unknown if destroyed)
• 5′ sequencing primer: AGCTGGTTTAGTGAACCGTCAGATC
• 3′ sequencing primer: TAATACGACTCACTATAGGG

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry