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Addgene

pBluescript II KS(+) EACMCV DNA-A 1.6mer
(Plasmid #159136)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159136 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II KS(+)
  • Vector type
    Infectious clone for bombarding plants

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EACMCV DNA-A 1.6mer
  • Species
    East African cassava mosaic Cameroon virus
  • Insert Size (bp)
    4348

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13 (-40)
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Clone originally described by Fondong et al. 2000 https://doi.org/10.1099/0022-1317-81-1-287

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBluescript II KS(+) EACMCV DNA-A 1.6mer was a gift from Vincent Fondong (Addgene plasmid # 159136 ; http://n2t.net/addgene:159136 ; RRID:Addgene_159136)

  • For your References section:

    Deeply Sequenced Infectious Clones of Key Cassava Begomovirus Isolates from Cameroon. Hoyer JS, Fondong VN, Dallas MM, Aimone CD, Deppong DO, Duffy S, Hanley-Bowdoin L. Microbiol Resour Announc. 2020 Nov 12;9(46). pii: 9/46/e00802-20. doi: 10.1128/MRA.00802-20. 10.1128/MRA.00802-20 PubMed 33184153
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