pUC19 ACMV DNA-B 1.5mer
(Plasmid #159135)

• Purpose: Infectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-B
• Depositing Lab: Vincent Fondong
• Publication: Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(46). pii: 9/46/e00802-20. doi: 10.1128/MRA.00802-20.

Backbone

• Vector backbone: pUC19
• Vector type: Infectious clone for bombarding plants

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ACMV DNA-B 1.5mer
• Species: African cassava mosaic virus
• Insert Size (bp): 4208

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: PstI (not destroyed)
• 5′ sequencing primer: M13 Reverse
• 3′ sequencing primer: M13 (-40)

Resource Information

• Supplemental Documents:
  • ab.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Clone originally described by Fondong et al. 2000 https://doi.org/10.1099/0022-1317-81-1-287

Plasmid is unstable, prone to truncation of the insert. Consider confirming size with a single-cutter such as BamHI.