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Addgene

RaCCR2_EYFP_pcDNA3.1
(Plasmid #159123)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159123 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RaCCR2
  • Alt name
    MN585303
  • Species
    Rhodomonas abbreviata
  • Insert Size (bp)
    948
  • Tag / Fusion Protein
    • EYFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RaCCR2_EYFP_pcDNA3.1 was a gift from John Spudich (Addgene plasmid # 159123 ; http://n2t.net/addgene:159123 ; RRID:Addgene_159123)
  • For your References section:

    Conductance Mechanisms of Rapidly Desensitizing Cation Channelrhodopsins from Cryptophyte Algae. Sineshchekov OA, Govorunova EG, Li H, Wang Y, Melkonian M, Wong GK, Brown LS, Spudich JL. mBio. 2020 Apr 21;11(2). pii: mBio.00657-20. doi: 10.1128/mBio.00657-20. 10.1128/mBio.00657-20 PubMed 32317325