RaCCR1_EYFP_pcDNA3.1
(Plasmid
#159122)
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PurposeThis plasmid encodes RaCCR1 rhodopsin domain
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159122 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRaCCR1
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Alt nameMN585300
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SpeciesRhodomonas abbreviata
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Insert Size (bp)993
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Tag
/ Fusion Protein
- EYFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RaCCR1_EYFP_pcDNA3.1 was a gift from John Spudich (Addgene plasmid # 159122 ; http://n2t.net/addgene:159122 ; RRID:Addgene_159122) -
For your References section:
Conductance Mechanisms of Rapidly Desensitizing Cation Channelrhodopsins from Cryptophyte Algae. Sineshchekov OA, Govorunova EG, Li H, Wang Y, Melkonian M, Wong GK, Brown LS, Spudich JL. mBio. 2020 Apr 21;11(2). pii: mBio.00657-20. doi: 10.1128/mBio.00657-20. 10.1128/mBio.00657-20 PubMed 32317325