CTV
(Plasmid #15912)

• Depositing Lab: Klaus Rajewsky
• Publication: Thai et al Science. 2007 Apr 27. 316(5824):604-8.

Backbone

• Vector backbone: pBSII KS
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2900
• Vector type: Cre/Lox ; /STOP
• Selectable markers: Neomycin (select with G418) ; Diptheriatoxin A

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Due to the large size of this plasmid, it takes longer to grow the bacteria to obtain sufficient amount of DNA to work with. I normally grow the bacteria at 37C in LB for 24-36 hours before extracting the plasmid.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CAG-STOP-eGFP-ROSA26TV
• Species: DNA derived from yeast, E. coli transposon Tn5 Corynebacterium aequoria (jellyfish)
• Insert Size (bp): 16103

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SnaBI (not destroyed)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: T7/M13
• 3′ sequencing primer: T7/M13

Resource Information

• Supplemental Documents:
  • CTV Vector Information
• Addgene Notes:
  • Restriction Digest of CTV
• A portion of this plasmid was derived from a plasmid made by: Based on STOP-eGFP-ROSA26TV by Klaus Rajewsky, IDI, Immune Disease Institute.
• Articles Citing this Plasmid:
  • 23 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map and attached PDF for more information.

The plasmid is also used in: Xiao C, Calado DP, Galler G, Thai TH, Patterson HC, Wang J, Rajewsky N, Bender TP, Rajewsky K. MiR-150 Controls B Cell Differentiation by Targeting the Transcription Factor c-Myb.
Cell. 2007 Oct 5;131(1):146-59.

Note that there are some differences between the author's theoretical sequence and Addgene's quality control sequence. The differences do not appear to be in critical regions required for plasmid function.