MvChR1_EYFP_pcDNA3.1
(Plasmid
#159119)
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PurposeThis plasmid encodes MvChR1 rhodopsin domain
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159119 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMvChR1
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Alt nameMChR1
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Alt nameJF922293
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SpeciesMesostigma viride
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Insert Size (bp)993
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Tag
/ Fusion Protein
- EYFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MvChR1_EYFP_pcDNA3.1 was a gift from John Spudich (Addgene plasmid # 159119 ; http://n2t.net/addgene:159119 ; RRID:Addgene_159119) -
For your References section:
New channelrhodopsin with a red-shifted spectrum and rapid kinetics from Mesostigma viride. Govorunova EG, Spudich EN, Lane CE, Sineshchekov OA, Spudich JL. mBio. 2011 Jun 21;2(3):e00115-11. doi: 10.1128/mBio.00115-11. Print 2011. 10.1128/mBio.00115-11 PubMed 21693637