pRSFDuet-sumo-NSP12 (SARS-CoV-2)
(Plasmid #159107)

• Purpose: The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).
• Depositing Lab: Thomas Tuschl
• Publication: Chen et al bioRxiv. 2020 Jul 13. doi: 10.1101/2020.07.08.194084.

Backbone

• Vector backbone: pRSFDuet-1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 6700
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NSP12
• Species: SARS-CoV-2
• Insert Size (bp): 2796
• Mutation: None
• Promoter: T7
• Tag / Fusion Protein:
  • His-SUMO tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-Term

Resource Information

• Supplemental Documents:
  • pRSFDuet-sumo-NSP12.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry