Skip to main content
Addgene

PB-CA tdTomato
(Plasmid #159095)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159095 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    PB-CA
  • Backbone manufacturer
    Andras Nagy
  • Vector type
    Mammalian Expression ; piggyBac

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tdTomato
  • Species
    Synthetic

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    piggyBac ITR's were from plasmid PB-CA (https://www.addgene.org/20960/). CAG promoter is from pCAGGS obtained from BCCM/LMBP. tdTomato is from Roger Tsien. WPRE is from Miguel Ramalho-Santos. bGH pA is from Neal Copeland.
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The tdTomato expression cassette insert is the same as in plasmid PB CAG-tdTomato (https://www.addgene.org/133569/)

Four nucleotides that were previously missing in the 5' end of the WPRE were added back, and SnapGene now recognizes it as WPRE.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-CA tdTomato was a gift from Mario Capecchi (Addgene plasmid # 159095 ; http://n2t.net/addgene:159095 ; RRID:Addgene_159095)
Related items:
Commonly requested with: