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PB-CA sfGFP
(Plasmid #159094)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159094 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB-CA
  • Backbone manufacturer
    Andras Nagy
  • Vector type
    Mammalian Expression ; piggyBac

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sfGFP
  • Species
    Synthetic

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    piggyBac ITR's were from plasmid PB-CA (https://www.addgene.org/20960/). CAG promoter is from pCAGGS obtained from BCCM/LMBP. sfGFP is from Sandia Biotech. WPRE is from Miguel Ramalho-Santos. bGH pA is from Neal Copeland.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sfGFP expression cassette insert is the same as in plasmid PB CAG-sfGFP (https://www.addgene.org/133568/)

Four nucleotides that were previously missing in the 5' end of the WPRE were added back, and SnapGene now recognizes it as WPRE.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-CA sfGFP was a gift from Mario Capecchi (Addgene plasmid # 159094 ; http://n2t.net/addgene:159094 ; RRID:Addgene_159094)
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