pCDFDuet-nsp7-nsp8
(Plasmid #159092)

• Purpose: Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8
• Depositing Lab: Seth Darst
• Publication: Chen et al bioRxiv. 2020 Jul 13. doi: 10.1101/2020.07.08.194084.

Backbone

• Vector backbone: pCDF Duet
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 4558
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Streptomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: NSP7
• Species: SARS-CoV-2 isolate Wuhan-Hu
• Insert Size (bp): 255
• GenBank ID: NC_045512.2
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: cgtcgacATGAGTAAGATGAGCGATGTTAAGTGTA

Gene/Insert 2

• Gene/Insert name: NSP8
• Species: SARS-CoV-2 isolate Wuhan-Hu
• Insert Size (bp): 600
• GenBank ID: NC_045512.2
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: tatacatATGGCGATTGCGTCAGAGTTCTC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry