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p5E-1.7kbfabp6
(Plasmid #159087)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159087 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p5E-Fse-Asc entry vector
  • Backbone manufacturer
    tol2kit
  • Vector type
    promoter fragment 5' entry vector for tol2 cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    1.7kb fabp6 promoter
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1700
  • Entrez Gene
    fabp6 (a.k.a. zgc:92421)
  • Promoter 1.7kb fabp6

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer ttaaggccggccGATGATCCCAACCCTGTAATGAGTTCCTGG
  • 3′ sequencing primer aattggcgcgccTTGAGAGCTGAGGTACTGATGGGTGAAG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5E-1.7kbfabp6 was a gift from John Rawls (Addgene plasmid # 159087 ; http://n2t.net/addgene:159087 ; RRID:Addgene_159087)

  • For your References section:

    Fxr signaling and microbial metabolism of bile salts in the zebrafish intestine. Wen J, Mercado GP, Volland A, Doden HL, Lickwar CR, Crooks T, Kakiyama G, Kelly C, Cocchiaro JL, Ridlon JM, Rawls JF. Sci Adv. 2021 Jul 23;7(30). pii: 7/30/eabg1371. doi: 10.1126/sciadv.abg1371. Print 2021 Jul. 10.1126/sciadv.abg1371 PubMed 34301599
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