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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15906 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRK5-myc
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCdc42 (placental isoform)
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Alt nameCdc42
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SpeciesH. sapiens (human)
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Insert Size (bp)600
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MutationQ61L
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Entrez GeneCDC42 (a.k.a. CDC42Hs, G25K, TKS)
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Tag
/ Fusion Protein
- myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Constitutively activated version of Cdc42 (G25K isoform)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK5myc Cdc42 L61 was a gift from Alan Hall (Addgene plasmid # 15906 ; http://n2t.net/addgene:15906 ; RRID:Addgene_15906) -
For your References section:
Rho GTPases control polarity, protrusion, and adhesion during cell movement. Nobes CD, Hall A. J Cell Biol. 1999 Mar 22. 144(6):1235-44. 10.1083/jcb.144.6.1235 PubMed 10087266